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Tender For purchase of chemicals - purchase of chemicals, diluent for dna extractionsize 500mla diluent used in dna extraction is typically te buffer (10 mm tris-hcl, 1 mm edta, ph ~8.0) or nuclease-free water, used to dissolve and dilute extracted dna. tris maintains a stable ph, while edta chelates divalent cations (e.g., mg ), thereby inhibiting dnase activity and protecting dna from degradation. the diluent must be sterile and free of nucleases to ensure the integrity of dna for downstream applications such as pcr, sequencing, and cloning., luria bertani mediumsize 500gluria bertani medium is a nutrient-rich growth medium commonly used for culturing bacteria such as escherichia coli and agrobacterium tumefaciens. it contains tryptone (10 g/l), yeast extract (5 g/l), and sodium chloride (10 g/l), which provide essential nutrients, vitamins, and osmotic balance for bacterial growth. lb medium supports rapid cell growth and is widely used in molecular biology for cloning, plasmid propagation, and transformation experiments. for solid media, agar (15g/l) is added., chloroform (high analytical reagent grade) size 2.5lchloroform (high analytical reagent grade) is a high-purity organic solvent used in dna extraction, particularly in phenol chloroform methods, to separate proteins and other contaminants from nucleic acids. it enhances phase separation by forming a distinct organic layer, allowing dna to remain in the aqueous phase. due to its toxicity and volatility, it must be handled under a fume hood with proper safety precautions., depcsize 25mldepc is a chemical used to inactivate rnases in water and laboratory solutions, making them suitable for rna work. it modifies histidine residues in proteins, thereby deactivating rnase enzymes that can degrade rna. depc-treated solutions are typically incubated and then autoclaved to remove residual depc, ensuring they are safe for use in downstream applications such as rna extraction and cdna synthesis., tb green master mixsize 200rnxtb green master mix is a ready-to-use reagent used in real-time pcr (qpcr) for dna amplification and detection. it contains tb green dye (a double-stranded dna-binding fluorescent dye), dna polymerase, dntps, mg , and reaction buffer. the dye binds to amplified double-stranded dna and emits fluorescence, allowing real-time monitoring of pcr amplification. it is commonly used for gene expression analysis and quantification of dna., dna loading dyesize 10 inchdna loading dye is a solution added to dna samples before agarose gel electrophoresis. it contains tracking dyes (such as bromophenol blue and xylene cyanol) that allow monitoring of dna migration during the run, and a dense agent (such as glycerol or sucrose) that helps the sample sink into the wells. it may also include edta to protect dna from nuclease degradation., metaloopa metal loop is a reusable laboratory tool made of platinum or nichrome wire, used for transferring and inoculating microorganisms onto culture media. it is sterilized by heating in a flame until red hot before and after use to prevent contamination. it is commonly used in microbiology for streaking plates and handling bacterial cultures.
Tender For supply of self recording rain gauge , maximun minimum thermometer digital , screw gauge , prismatic compass with stand , auto dumpy level with stand and staff , abney level , robotic total station 1 inch accuracy bluetooth auto target , kerosene or gas stove or electric hot plate , ghat tracer , pegging rod , optical square 90 degree , ranging rod 3 mtr long , high precision binacular , temperature gun for checking bituminous surface works , sample tray enamelled , vibrating wire , termperature sensor , portable indicator , steel tape 5m , standard pyconometer of 50 ml , digital hygrometer , post hole auger with extensions , rodo meter - high precision wheel odometer , hot plates 200 mm dia 1500 watt voltage 220v to 230v , circular plates of 250 mm dia , sampling pipettes fitted with pressure and suction inlets, 10 ml capacity. , sampling tins with lids 100 mm dia x 75 mm ht 500 gms capacity , electronic digital micrometer , ennore standard sand 25 kgs bag , weighing machine 0 kg to 10 kgs , mould 15cmx15cmx15cm for compressive strength of concrete , digital vernier calipers accuracy 0.001 mm , thermometers mercuryinglass thermometer 0 to 250 c mercury-in-steel , glass thermometers range 0 degree c to 100 degree c 100 degree c , mortar cube 70 pt 6 mm , cbr plunger with settlement dial gauge holder , ring and ball app for softening point , specific gravity test bottle with vaccum pump , tripod stands for holding dial gauge holder , metallic laboratory thermometers range up to 300 c , spacers disc 148 mm dia 47.7 mm ht. with handle , comprising beakers capacity 1000 ml glassware , pipettes capacity 50 ml glassware , perforated plate brass , metallic thermometers range up to 300degree c 300 degree c , specific gravity and water absorption testing machine , marshall sample mould , stop watch one and half sec. accuracy , field density apparutus complete setsand relacment method , compaction test equipment both 2.5 kg and 4.5 kg rammers , cbr moulds 150 mm dia -175 mm ht. , 150 mm x 300 mm ht. cylinder with capping component , riffle box , soaking tank for accommodating 6 cbr moulds , speedy moisture meter complete with chemicals , standard measures of 30, 15 and 3 litres capacity alongwith standard tamping rod , distant reading thermometer 0 degree c 250 degree c , sand pouring cylinder with conical funnel and tap and complete , gi seive set 125,90,75, 63,53,45,37.5, 14, 13.2,12.5,11.2,10,9.5,5.6,4.75mm , water still 20 ltr capacity , glass beakers borosilicate 4 nos of 100 cc, 200 cc, 500cc, 1000cc capacity , pentrometer with standard needles , platform balance 300 kg capacity , drying ovens. thermostatically controlled 100 degree c to 110 degree , water bath electrically operated and thermostatically controlled , dial gauge 25 mm travel -0.01 mm or division , aiv testing apparatus complete set , is sieve sets 450 mm internal dia with lid and pan 30 sieves in 1 set , sieves 450 mm internal dia of sieve sets as per bis of required sieve sizes , is brass sieve sets 200 mm internal dia with lid and pan 35 sieves in 1 set , sieves 200 mm consisting of sieve set of required sieve sizes with lid and pan , is sieve 75,53,45,22.4,11.22 6.5,9.50,5.6,4.75,2.36,1.18mm , soxhlet extraction or centrifuge type apparatus , oven electrically operated, thermostatically controlled including thermometer , sieve shaker capable of shaking 200 mm operated with time switch , weight machine 30 kg electronic type , high frequency mortar cube vibrator for cement testing , specific gravityapparatus , rapid moisture meter is 12175 1987 , flash and fire point apparatus , ductility testing apparatus electrically operated , density testing machine complete set bucket method , rebound hammer concrete testing hammer , furol viscometer , sand equivalent test apparatus , standard sand for density test , lab cbr testing equipment for conducting cbr testing 5 tonne cap , unconfined compression test appratus , marshal stability test machine , los-angeles abrasion te
Tender For corrigendum : sch 5 drugs, pharmaceutical and disinfectants by tender - schedule v (drugs, pharmaceuticals & disinfectant), carbolic (phenol crystal) acid, ammonium chloride, borax, crystal violet medicinal use, emulsifying wax, ether solvent, methyl salicylate, paraffin soft white, potassium chloride, potassium iodide, soda lime granules medical grade for anaesthesia purpose. non-hygroscopic . granules size 4-8 mesh size. suitable indication with change of colour to denote exhaustion of granules. (rate to be quoted for 1 x 5 kg tin) i.p., sodium bicarbonate, calamine prepared, clove oil, soap solution i. s. i. mark, antiseptic solution containing benzalkonium chloride solution 20% w/v equivalent to benzalkonium chloride equivalent to 10%w/v, 70% to 80% alcohol based surgical dry hand antiseptic with 0.5% to 1% chlorhexidine gluconate and containing suitable emollient and moisturizer., 70% to 80% alcohol based surgical dry hand antiseptic with a substance included in i.p.other than chlorhexidine gluconate having a broad spectrum activity against bacteria, virus (inclusive hiv and hbv) and fungi with suitable emollient and moisturizer., chlorhexidine (not less than 1.5% w./ v. or 7.5 % v/v) with cetrimide 15%, povidone iodine i.p. should contain povidone iodine 10% (available iodine 1% w/v) (rate to be quoted for 1000ml), solution containing > 2 % w/v glutaraldehyde with a powder activator free of surfactants with stable alkaline ph for 14 days with concentration detections strips(10 strips/5lit jar.), surface & environmental disinfectant each 100 gm containing 1.6 dihydroxy, 2.5 diohexane 11.2gm, gluteraldehyde 5gm benzalkonium chloride 5gm alkyl urear derivatives 3.00gm ( effectivity reports against hbv/hiv & t.b. is necessary), surface and environmental disinfectant: aldehyde free disinfec- tant with bacetericidal, fungicidal, virucidal(including hiv and hbv) and sporicidal activity capable of being used as both surface and environmental disinfectant as well as for fogging. the product label should mention the shelf life also., sodium chloride (i.p.), opa orthophthalaldehyde 0.55% w/w with marker technology with detection strips (5 test strips/5 lit pack), enzymatic instrument cleaner multienzymatic enzymatic cleaner, neutral ph, compatible for use on surgical, medical and dental instruments with atleast protease ,amylase,& lipase enzyme activity (rate to be quoted per ltr 1 x 1 ltrs) (supply in suitable packing upto 5 lit. jar), denaturated spirit 95-97% ordinary, analgesic transdermal patch fentanyl 25mcg/hr, adhesive remover spray 100 % silicon, 1 % acetic acid 500 ml bottle, methylene blue, na fluorescence strips, molecular grade water, lint free tissue
Tender For bid to ras supply of is test sieve of aperture size 10mm, 4.75mm, 2.36mm, 1.18mm, 600mic, 300mic, 150mic, 90mic in 20 cm dia. as per specification documents. , pan and cover of 20 cm dia is sieve. as per specification documents. , is test sieve of aperture different sizes. as per specification documents. , sieve pan size 45 and 20 cm in dia. as per specification documents. , electronic weighing balance machine 20 kgs cap. as per specification documents. , enamel tray size 45cm x 30cm x 4cm. as per specification documents. , enamel tray size 30cm x 25cm x 4cm. as per specification documents. , bitumen centrifuge extractor electric operated. as per specification documents. , benzene packed in glass pack of 2.5 ltrs. as per specification documents. , standard sand for the sand replacement method, field density test. as per specification documents. , parafin wax for density of dbm and bc. as per specification documents. , vernier calipers 150 x 0.1mm digital , core cutting machine. as per specification documents. , ring and ball apparatus, digital. as per specification documents. , marshal mould of size 101.6 mm dia inside x 76.2 mm height. as per specification documents. , marshall mould filter paper should have 100 mm dia 1.0 mm thickness. pkt of 100 circles , bitumen extractor filter paper. as per specification documents. , specific gravity bottle of 50 ml capacity. as per specification documents. , kerosene pressure stove should have 3.00 liter tank capacity fitted with single 4 no burner. , standard spatula made of stainless steel blade with wooden handle approx. 300 mm length. as per specification documents. , steel straight edge. as per specification documents. , standard le chatelier flask, soundness test apparatus. as per specification documents. , test sieve 75 micron ss mesh with brass frame 200 mm dia. as per specification documents. , test sieve 90 micron ss mesh with brass frame 200 mm dia. as per specification documents. , ordinary mercury-in-glass thermometer designed for the 0 to 60 degree range. as per specification documents. , ordinary mercury-in-glass thermometer designed for the 0 to 100 degree range. as per specification documents. , ordinary mercury-in-glass thermometer designed for the 0 to 360 degree range. as per specification documents. , nitrile rubber gloves for laboratory use. as per specification documents. , cotton cloth for laboratory use. as per specification documents. , stainless steel flakiness index gauge. as per specification documents. , stainless steel elongation index gauge mounted on wooden block. as per specification documents. , digital electronic weighing machine max 3 kg capacity. as per specification documents. , cast iron cube moulds with base plate size 15 x 15 x 15 cms. as per specification documents. , diamond core bits for core cutting machine. as per specification documents. , measuring cylinder capacity 50ml, 100ml, 250ml and 500ml each. as per specification documents. , specific gravityapparatus, buoyancy balance test apparatus. as per specification documents.